Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM05-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM05-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 121477726 Number of paired-end alignments with a unique best hit: 8664305 Mapping efficiency: 7.1% Sequence pairs with no alignments under any condition: 108244447 Sequence pairs did not map uniquely: 4568974 Sequence pairs which were discarded because genomic sequence could not be extracted: 16 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2257139 ((converted) top strand) GA/CT/CT: 2069633 (complementary to (converted) top strand) GA/CT/GA: 2073793 (complementary to (converted) bottom strand) CT/GA/GA: 2263724 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 198057902 Total methylated C's in CpG context: 3391868 Total methylated C's in CHG context: 893353 Total methylated C's in CHH context: 5515515 Total methylated C's in Unknown context: 260010 Total unmethylated C's in CpG context: 22417388 Total unmethylated C's in CHG context: 36063675 Total unmethylated C's in CHH context: 129776103 Total unmethylated C's in Unknown context: 1878448 C methylated in CpG context: 13.1% C methylated in CHG context: 2.4% C methylated in CHH context: 4.1% C methylated in unknown context (CN or CHN): 12.2% Bismark completed in 0d 3h 45m 38s