Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM01-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM01-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 106485421 Number of paired-end alignments with a unique best hit: 30408684 Mapping efficiency: 28.6% Sequence pairs with no alignments under any condition: 64271971 Sequence pairs did not map uniquely: 11804766 Sequence pairs which were discarded because genomic sequence could not be extracted: 20 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7386387 ((converted) top strand) GA/CT/CT: 7788869 (complementary to (converted) top strand) GA/CT/GA: 7815962 (complementary to (converted) bottom strand) CT/GA/GA: 7417446 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1229090290 Total methylated C's in CpG context: 36033631 Total methylated C's in CHG context: 6119258 Total methylated C's in CHH context: 23280204 Total methylated C's in Unknown context: 2076417 Total unmethylated C's in CpG context: 152463973 Total unmethylated C's in CHG context: 260564146 Total unmethylated C's in CHH context: 750629078 Total unmethylated C's in Unknown context: 6415161 C methylated in CpG context: 19.1% C methylated in CHG context: 2.3% C methylated in CHH context: 3.0% C methylated in unknown context (CN or CHN): 24.5% Bismark completed in 0d 5h 33m 59s