Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF04-EM05-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF04-EM05-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 141036792 Number of paired-end alignments with a unique best hit: 50570557 Mapping efficiency: 35.9% Sequence pairs with no alignments under any condition: 71629128 Sequence pairs did not map uniquely: 18837107 Sequence pairs which were discarded because genomic sequence could not be extracted: 59 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11911693 ((converted) top strand) GA/CT/CT: 13346218 (complementary to (converted) top strand) GA/CT/GA: 13372715 (complementary to (converted) bottom strand) CT/GA/GA: 11939872 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2122638215 Total methylated C's in CpG context: 54639895 Total methylated C's in CHG context: 7385773 Total methylated C's in CHH context: 28959741 Total methylated C's in Unknown context: 1987381 Total unmethylated C's in CpG context: 263409541 Total unmethylated C's in CHG context: 446941021 Total unmethylated C's in CHH context: 1321302244 Total unmethylated C's in Unknown context: 10026806 C methylated in CpG context: 17.2% C methylated in CHG context: 1.6% C methylated in CHH context: 2.1% C methylated in unknown context (CN or CHN): 16.5% Bismark completed in 0d 15h 24m 29s