Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF03-EM04-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF03-EM04-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 133115292 Number of paired-end alignments with a unique best hit: 40445897 Mapping efficiency: 30.4% Sequence pairs with no alignments under any condition: 77039566 Sequence pairs did not map uniquely: 15629829 Sequence pairs which were discarded because genomic sequence could not be extracted: 33 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11089506 ((converted) top strand) GA/CT/CT: 9093581 (complementary to (converted) top strand) GA/CT/GA: 9125385 (complementary to (converted) bottom strand) CT/GA/GA: 11137392 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1485144673 Total methylated C's in CpG context: 46571077 Total methylated C's in CHG context: 16714377 Total methylated C's in CHH context: 61901138 Total methylated C's in Unknown context: 2684181 Total unmethylated C's in CpG context: 164519313 Total unmethylated C's in CHG context: 298094084 Total unmethylated C's in CHH context: 897344684 Total unmethylated C's in Unknown context: 8999478 C methylated in CpG context: 22.1% C methylated in CHG context: 5.3% C methylated in CHH context: 6.5% C methylated in unknown context (CN or CHN): 23.0% Bismark completed in 0d 5h 8m 59s