Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF03-EM03-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF03-EM03-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 135271836 Number of paired-end alignments with a unique best hit: 22016467 Mapping efficiency: 16.3% Sequence pairs with no alignments under any condition: 103804735 Sequence pairs did not map uniquely: 9450634 Sequence pairs which were discarded because genomic sequence could not be extracted: 21 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6178649 ((converted) top strand) GA/CT/CT: 4793827 (complementary to (converted) top strand) GA/CT/GA: 4822688 (complementary to (converted) bottom strand) CT/GA/GA: 6221282 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 581355521 Total methylated C's in CpG context: 15352387 Total methylated C's in CHG context: 5077870 Total methylated C's in CHH context: 25138967 Total methylated C's in Unknown context: 1140538 Total unmethylated C's in CpG context: 56233493 Total unmethylated C's in CHG context: 104518461 Total unmethylated C's in CHH context: 375034343 Total unmethylated C's in Unknown context: 4116576 C methylated in CpG context: 21.4% C methylated in CHG context: 4.6% C methylated in CHH context: 6.3% C methylated in unknown context (CN or CHN): 21.7% Bismark completed in 0d 4h 2m 45s