Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF02-EM02-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF02-EM02-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 144270632 Number of paired-end alignments with a unique best hit: 33068786 Mapping efficiency: 22.9% Sequence pairs with no alignments under any condition: 95502188 Sequence pairs did not map uniquely: 15699658 Sequence pairs which were discarded because genomic sequence could not be extracted: 58 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9370832 ((converted) top strand) GA/CT/CT: 7241324 (complementary to (converted) top strand) GA/CT/GA: 7168801 (complementary to (converted) bottom strand) CT/GA/GA: 9287771 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1069984067 Total methylated C's in CpG context: 24195692 Total methylated C's in CHG context: 4608455 Total methylated C's in CHH context: 21726525 Total methylated C's in Unknown context: 1445840 Total unmethylated C's in CpG context: 132851049 Total unmethylated C's in CHG context: 221787521 Total unmethylated C's in CHH context: 664814825 Total unmethylated C's in Unknown context: 7438716 C methylated in CpG context: 15.4% C methylated in CHG context: 2.0% C methylated in CHH context: 3.2% C methylated in unknown context (CN or CHN): 16.3% Bismark completed in 0d 4h 41m 27s