Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM05-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM05-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 80683717 Number of paired-end alignments with a unique best hit: 1716695 Mapping efficiency: 2.1% Sequence pairs with no alignments under any condition: 78416271 Sequence pairs did not map uniquely: 550751 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 439115 ((converted) top strand) GA/CT/CT: 418914 (complementary to (converted) top strand) GA/CT/GA: 418486 (complementary to (converted) bottom strand) CT/GA/GA: 440180 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 73983804 Total methylated C's in CpG context: 1506054 Total methylated C's in CHG context: 237171 Total methylated C's in CHH context: 1750182 Total methylated C's in Unknown context: 98340 Total unmethylated C's in CpG context: 7890781 Total unmethylated C's in CHG context: 13552439 Total unmethylated C's in CHH context: 49047177 Total unmethylated C's in Unknown context: 339559 C methylated in CpG context: 16.0% C methylated in CHG context: 1.7% C methylated in CHH context: 3.4% C methylated in unknown context (CN or CHN): 22.5% Bismark completed in 0d 2h 57m 33s