Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM04-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM04-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53999416 Number of paired-end alignments with a unique best hit: 578096 Mapping efficiency: 1.1% Sequence pairs with no alignments under any condition: 53163382 Sequence pairs did not map uniquely: 257938 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 156481 ((converted) top strand) GA/CT/CT: 134587 (complementary to (converted) top strand) GA/CT/GA: 131526 (complementary to (converted) bottom strand) CT/GA/GA: 155502 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 19838721 Total methylated C's in CpG context: 440333 Total methylated C's in CHG context: 102279 Total methylated C's in CHH context: 1773097 Total methylated C's in Unknown context: 56096 Total unmethylated C's in CpG context: 1900494 Total unmethylated C's in CHG context: 3354118 Total unmethylated C's in CHH context: 12268400 Total unmethylated C's in Unknown context: 110289 C methylated in CpG context: 18.8% C methylated in CHG context: 3.0% C methylated in CHH context: 12.6% C methylated in unknown context (CN or CHN): 33.7% Bismark completed in 0d 1h 29m 1s