Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM03-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF08-CM03-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 147000143 Number of paired-end alignments with a unique best hit: 27270023 Mapping efficiency: 18.6% Sequence pairs with no alignments under any condition: 108690029 Sequence pairs did not map uniquely: 11040091 Sequence pairs which were discarded because genomic sequence could not be extracted: 30 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8097427 ((converted) top strand) GA/CT/CT: 5517413 (complementary to (converted) top strand) GA/CT/GA: 5527808 (complementary to (converted) bottom strand) CT/GA/GA: 8127345 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 686635733 Total methylated C's in CpG context: 15769913 Total methylated C's in CHG context: 4596366 Total methylated C's in CHH context: 27966021 Total methylated C's in Unknown context: 1028546 Total unmethylated C's in CpG context: 64879675 Total unmethylated C's in CHG context: 118811730 Total unmethylated C's in CHH context: 454612028 Total unmethylated C's in Unknown context: 4664252 C methylated in CpG context: 19.6% C methylated in CHG context: 3.7% C methylated in CHH context: 5.8% C methylated in unknown context (CN or CHN): 18.1% Bismark completed in 0d 4h 40m 5s