Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF06-CM02-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF06-CM02-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 113129286 Number of paired-end alignments with a unique best hit: 24333652 Mapping efficiency: 21.5% Sequence pairs with no alignments under any condition: 80016444 Sequence pairs did not map uniquely: 8779190 Sequence pairs which were discarded because genomic sequence could not be extracted: 10 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6207843 ((converted) top strand) GA/CT/CT: 5906395 (complementary to (converted) top strand) GA/CT/GA: 5956795 (complementary to (converted) bottom strand) CT/GA/GA: 6262609 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 837836621 Total methylated C's in CpG context: 28354876 Total methylated C's in CHG context: 9997804 Total methylated C's in CHH context: 42244001 Total methylated C's in Unknown context: 1614056 Total unmethylated C's in CpG context: 87667939 Total unmethylated C's in CHG context: 163824422 Total unmethylated C's in CHH context: 505747579 Total unmethylated C's in Unknown context: 5039881 C methylated in CpG context: 24.4% C methylated in CHG context: 5.8% C methylated in CHH context: 7.7% C methylated in unknown context (CN or CHN): 24.3% Bismark completed in 0d 3h 56m 20s