Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF06-CM01-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF06-CM01-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 148365024 Number of paired-end alignments with a unique best hit: 34754399 Mapping efficiency: 23.4% Sequence pairs with no alignments under any condition: 100017007 Sequence pairs did not map uniquely: 13593618 Sequence pairs which were discarded because genomic sequence could not be extracted: 60 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9523000 ((converted) top strand) GA/CT/CT: 7783662 (complementary to (converted) top strand) GA/CT/GA: 7849305 (complementary to (converted) bottom strand) CT/GA/GA: 9598372 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 984629170 Total methylated C's in CpG context: 28037772 Total methylated C's in CHG context: 7747165 Total methylated C's in CHH context: 37244986 Total methylated C's in Unknown context: 1730942 Total unmethylated C's in CpG context: 100998783 Total unmethylated C's in CHG context: 185411768 Total unmethylated C's in CHH context: 625188696 Total unmethylated C's in Unknown context: 6621912 C methylated in CpG context: 21.7% C methylated in CHG context: 4.0% C methylated in CHH context: 5.6% C methylated in unknown context (CN or CHN): 20.7% Bismark completed in 0d 4h 50m 50s