Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF04-CM04-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF04-CM04-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 129476805 Number of paired-end alignments with a unique best hit: 32682901 Mapping efficiency: 25.2% Sequence pairs with no alignments under any condition: 84478802 Sequence pairs did not map uniquely: 12315102 Sequence pairs which were discarded because genomic sequence could not be extracted: 39 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9423131 ((converted) top strand) GA/CT/CT: 6939040 (complementary to (converted) top strand) GA/CT/GA: 6919178 (complementary to (converted) bottom strand) CT/GA/GA: 9401513 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1052320771 Total methylated C's in CpG context: 26836356 Total methylated C's in CHG context: 5919122 Total methylated C's in CHH context: 28554690 Total methylated C's in Unknown context: 1517232 Total unmethylated C's in CpG context: 113192371 Total unmethylated C's in CHG context: 205379824 Total unmethylated C's in CHH context: 672438408 Total unmethylated C's in Unknown context: 6030986 C methylated in CpG context: 19.2% C methylated in CHG context: 2.8% C methylated in CHH context: 4.1% C methylated in unknown context (CN or CHN): 20.1% Bismark completed in 0d 4h 31m 49s