Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF03-CM05-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF03-CM05-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 114639363 Number of paired-end alignments with a unique best hit: 38253850 Mapping efficiency: 33.4% Sequence pairs with no alignments under any condition: 61987067 Sequence pairs did not map uniquely: 14398446 Sequence pairs which were discarded because genomic sequence could not be extracted: 28 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9359769 ((converted) top strand) GA/CT/CT: 9722903 (complementary to (converted) top strand) GA/CT/GA: 9774279 (complementary to (converted) bottom strand) CT/GA/GA: 9396871 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1552465266 Total methylated C's in CpG context: 45560778 Total methylated C's in CHG context: 11557139 Total methylated C's in CHH context: 42165956 Total methylated C's in Unknown context: 2438938 Total unmethylated C's in CpG context: 189329253 Total unmethylated C's in CHG context: 326079297 Total unmethylated C's in CHH context: 937772843 Total unmethylated C's in Unknown context: 8182791 C methylated in CpG context: 19.4% C methylated in CHG context: 3.4% C methylated in CHH context: 4.3% C methylated in unknown context (CN or CHN): 23.0% Bismark completed in 0d 4h 45m 56s