Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF03-CM03-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF03-CM03-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 131905267 Number of paired-end alignments with a unique best hit: 32406119 Mapping efficiency: 24.6% Sequence pairs with no alignments under any condition: 86434679 Sequence pairs did not map uniquely: 13064469 Sequence pairs which were discarded because genomic sequence could not be extracted: 22 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9116347 ((converted) top strand) GA/CT/CT: 7059721 (complementary to (converted) top strand) GA/CT/GA: 7074390 (complementary to (converted) bottom strand) CT/GA/GA: 9155639 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 913610847 Total methylated C's in CpG context: 24525893 Total methylated C's in CHG context: 5617169 Total methylated C's in CHH context: 30119527 Total methylated C's in Unknown context: 1364370 Total unmethylated C's in CpG context: 94463595 Total unmethylated C's in CHG context: 176142092 Total unmethylated C's in CHH context: 582742571 Total unmethylated C's in Unknown context: 5419248 C methylated in CpG context: 20.6% C methylated in CHG context: 3.1% C methylated in CHH context: 4.9% C methylated in unknown context (CN or CHN): 20.1% Bismark completed in 0d 4h 21m 59s