Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF02-CM02-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF02-CM02-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 207947580 Number of paired-end alignments with a unique best hit: 28344779 Mapping efficiency: 13.6% Sequence pairs with no alignments under any condition: 167753891 Sequence pairs did not map uniquely: 11848910 Sequence pairs which were discarded because genomic sequence could not be extracted: 42 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8584018 ((converted) top strand) GA/CT/CT: 5553562 (complementary to (converted) top strand) GA/CT/GA: 5572591 (complementary to (converted) bottom strand) CT/GA/GA: 8634566 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 671751469 Total methylated C's in CpG context: 15992068 Total methylated C's in CHG context: 6238333 Total methylated C's in CHH context: 35965711 Total methylated C's in Unknown context: 1087764 Total unmethylated C's in CpG context: 61863172 Total unmethylated C's in CHG context: 113759888 Total unmethylated C's in CHH context: 437932297 Total unmethylated C's in Unknown context: 4742286 C methylated in CpG context: 20.5% C methylated in CHG context: 5.2% C methylated in CHH context: 7.6% C methylated in unknown context (CN or CHN): 18.7% Bismark completed in 0d 5h 58m 49s