Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF01-CM01-Zygote_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/CF01-CM01-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 32085370 Number of paired-end alignments with a unique best hit: 4232230 Mapping efficiency: 13.2% Sequence pairs with no alignments under any condition: 26181125 Sequence pairs did not map uniquely: 1672015 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1143966 ((converted) top strand) GA/CT/CT: 974626 (complementary to (converted) top strand) GA/CT/GA: 971605 (complementary to (converted) bottom strand) CT/GA/GA: 1142028 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 118915438 Total methylated C's in CpG context: 2668090 Total methylated C's in CHG context: 562060 Total methylated C's in CHH context: 3117889 Total methylated C's in Unknown context: 168999 Total unmethylated C's in CpG context: 12183733 Total unmethylated C's in CHG context: 21440246 Total unmethylated C's in CHH context: 78943420 Total unmethylated C's in Unknown context: 823411 C methylated in CpG context: 18.0% C methylated in CHG context: 2.6% C methylated in CHH context: 3.8% C methylated in unknown context (CN or CHN): 17.0% Bismark completed in 0d 0h 57m 22s