## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[S7M.sorted.bam] OUTPUT=../output/11-RNAseq-snps/S7M.dedup.bam METRICS_FILE=../output/11-RNAseq-snps/S7M.dup_metrics.txt REMOVE_DUPLICATES=true MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=2 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Mar 09 13:23:20 PST 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 3054568 13485124 16838036 21087920 388564 606880 52755 0.053367 158295968 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.003763 12346379 0 12395280 2.0 1.925556 501024 52202 455526 3.0 2.772075 22388 273 19182 4.0 3.549465 3156 1 3001 5.0 4.263373 1442 1 1434 6.0 4.918982 881 0 862 7.0 5.521052 570 0 566 8.0 6.073957 396 0 395 9.0 6.581711 322 0 325 10.0 7.048 239 0 235 11.0 7.476212 195 0 194 12.0 7.869455 143 0 140 13.0 8.230586 120 0 122 14.0 8.562226 108 0 106 15.0 8.866783 88 0 90 16.0 9.14647 67 0 63 17.0 9.403318 57 0 59 18.0 9.63919 73 0 71 19.0 9.855801 64 0 65 20.0 10.054724 37 0 36 21.0 10.237402 37 0 38 22.0 10.405162 31 0 32 23.0 10.559222 38 0 38 24.0 10.700702 29 0 24 25.0 10.830628 23 0 24 26.0 10.949945 20 0 22 27.0 11.059517 23 0 24 28.0 11.160142 23 0 20 29.0 11.25255 15 0 16 30.0 11.337412 16 0 16 31.0 11.415343 11 0 9 32.0 11.486911 16 0 17 33.0 11.552634 14 0 13 34.0 11.61299 16 0 17 35.0 11.668418 5 0 7 36.0 11.719319 14 0 14 37.0 11.766063 8 0 7 38.0 11.80899 14 0 12 39.0 11.848412 7 0 7 40.0 11.884615 7 0 9 41.0 11.917861 11 0 9 42.0 11.948392 6 0 8 43.0 11.97643 6 0 4 44.0 12.002178 8 0 8 45.0 12.025824 7 0 8 46.0 12.047539 5 0 5 47.0 12.06748 4 0 3 48.0 12.085793 2 0 3 49.0 12.102611 6 0 5 50.0 12.118055 2 0 2 51.0 12.132238 3 0 3 52.0 12.145263 2 0 2 53.0 12.157224 2 0 3 54.0 12.168208 2 0 2 55.0 12.178296 2 0 3 56.0 12.187559 4 0 4 57.0 12.196067 5 0 4 58.0 12.203879 1 0 0 59.0 12.211054 1 0 1 60.0 12.217642 1 0 2 61.0 12.223693 2 0 1 62.0 12.229249 4 0 5 63.0 12.234352 2 0 1 64.0 12.239038 3 0 3 65.0 12.243341 2 0 2 66.0 12.247293 0 0 0 67.0 12.250923 0 0 0 68.0 12.254255 3 0 3 69.0 12.257316 2 0 2 70.0 12.260127 0 0 0 71.0 12.262708 1 0 1 72.0 12.265078 2 0 2 73.0 12.267255 0 0 0 74.0 12.269254 0 0 0 75.0 12.27109 0 0 1 76.0 12.272776 1 0 1 77.0 12.274324 1 0 0 78.0 12.275746 1 0 1 79.0 12.277052 0 0 0 80.0 12.278251 1 0 1 81.0 12.279352 0 0 0 82.0 12.280363 0 0 0 83.0 12.281292 1 0 1 84.0 12.282145 1 0 1 85.0 12.282928 0 0 0 86.0 12.283647 0 0 0 87.0 12.284308 0 0 0 88.0 12.284914 1 0 1 89.0 12.285471 0 0 0 90.0 12.285983 1 0 1 91.0 12.286453 0 0 0 92.0 12.286884 0 0 1 93.0 12.28728 1 0 0 94.0 12.287644 0 0 0 95.0 12.287978 0 0 0 96.0 12.288285 0 0 0 97.0 12.288567 0 0 0 98.0 12.288825 0 0 0 99.0 12.289063 0 0 0 100.0 12.289281 0 0 0 102.0 0 1 0 1 105.0 0 0 0 1 106.0 0 2 0 1 108.0 0 0 0 1 109.0 0 1 0 1 111.0 0 2 0 2 112.0 0 1 0 0 114.0 0 4 0 4 117.0 0 1 0 2 119.0 0 1 0 0 127.0 0 0 0 1 128.0 0 1 0 0 130.0 0 1 0 1 133.0 0 0 0 1 134.0 0 1 0 0 169.0 0 0 0 1 170.0 0 1 0 0 238.0 0 0 0 1 242.0 0 1 0 0