## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[S31M.sorted.bam] OUTPUT=../output/11-RNAseq-snps/S31M.dedup.bam METRICS_FILE=../output/11-RNAseq-snps/S31M.dup_metrics.txt REMOVE_DUPLICATES=true MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=2 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Mar 09 11:50:59 PST 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 2349657 10934543 13615835 15403793 275213 504106 40844 0.052993 124426189 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.003587 9951767 0 9989224 2.0 1.922743 457036 40404 422726 3.0 2.764572 19801 217 16873 4.0 3.535578 1241 2 1030 5.0 4.24172 242 0 240 6.0 4.888456 130 0 126 7.0 5.480782 75 0 73 8.0 6.023276 34 0 34 9.0 6.520131 35 0 35 10.0 6.975186 9 0 9 11.0 7.391957 14 0 14 12.0 7.773666 11 0 11 13.0 8.123263 9 0 10 14.0 8.443448 7 0 6 15.0 8.736696 0 0 0 16.0 9.005273 3 0 3 17.0 9.251256 2 0 3 18.0 9.476544 4 0 3 19.0 9.682879 4 0 4 20.0 9.871855 1 0 1 21.0 10.044932 1 0 1 22.0 10.203449 3 0 3 23.0 10.34863 0 0 0 24.0 10.481597 0 0 0 25.0 10.603378 2 0 2 26.0 10.714913 0 0 0 27.0 10.817066 0 0 1 28.0 10.910624 3 0 2 29.0 10.996311 1 0 1 30.0 11.074789 0 0 0 31.0 11.146665 0 0 0 32.0 11.212494 0 0 0 33.0 11.272785 0 0 0 34.0 11.328004 0 0 0 35.0 11.378578 0 0 0 36.0 11.424896 0 0 0 37.0 11.467318 0 0 0 38.0 11.506171 0 0 0 39.0 11.541755 0 0 0 40.0 11.574346 0 0 0 41.0 11.604195 0 0 0 42.0 11.631533 0 0 0 43.0 11.65657 0 0 0 44.0 11.679502 0 0 0 45.0 11.700504 0 0 0 46.0 11.719739 0 0 0 47.0 11.737356 0 0 0 48.0 11.753491 0 0 0 49.0 11.768269 0 0 0 50.0 11.781803 0 0 0 51.0 11.794199 0 0 0 52.0 11.805552 0 0 0 53.0 11.815949 0 0 0 54.0 11.825472 0 0 0 55.0 11.834194 0 0 0 56.0 11.842182 0 0 0 57.0 11.849498 0 0 0 58.0 11.856199 0 0 0 59.0 11.862336 0 0 0 60.0 11.867956 0 0 0 61.0 11.873104 0 0 0 62.0 11.877819 0 0 0 63.0 11.882137 0 0 0 64.0 11.886091 0 0 0 65.0 11.889713 0 0 0 66.0 11.893031 0 0 0 67.0 11.896069 0 0 1 68.0 11.898851 1 0 0 69.0 11.9014 0 0 0 70.0 11.903734 0 0 0 71.0 11.905872 0 0 0 72.0 11.90783 0 0 0 73.0 11.909623 0 0 0 74.0 11.911265 0 0 0 75.0 11.912769 0 0 1 76.0 11.914147 1 0 0 77.0 11.915409 0 0 0 78.0 11.916564 0 0 0 79.0 11.917623 0 0 0 80.0 11.918592 0 0 0 81.0 11.91948 0 0 0 82.0 11.920293 0 0 0 83.0 11.921037 0 0 0 84.0 11.921719 0 0 0 85.0 11.922344 0 0 0 86.0 11.922916 0 0 0 87.0 11.92344 0 0 0 88.0 11.92392 0 0 0 89.0 11.92436 0 0 0 90.0 11.924762 0 0 0 91.0 11.925131 0 0 0 92.0 11.925468 0 0 0 93.0 11.925778 0 0 0 94.0 11.926061 0 0 0 95.0 11.92632 0 0 0 96.0 11.926558 0 0 0 97.0 11.926776 0 0 0 98.0 11.926975 0 0 0 99.0 11.927157 0 0 0 100.0 11.927325 0 0 0