## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[S12M.sorted.bam] OUTPUT=../output/11-RNAseq-snps/S12M.dedup.bam METRICS_FILE=../output/11-RNAseq-snps/S12M.dup_metrics.txt REMOVE_DUPLICATES=true MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=2 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Mar 09 11:11:23 PST 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 3671993 15654415 18592978 25235299 500715 722750 69010 0.055637 180548639 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.004239 14260545 0 14324418 2.0 1.925073 639018 68360 579857 3.0 2.76943 27087 319 22645 4.0 3.543662 2367 4 2132 5.0 4.253592 791 0 779 6.0 4.90456 475 0 467 7.0 5.501465 288 0 283 8.0 6.048795 213 0 213 9.0 6.550668 138 0 132 10.0 7.01086 108 0 111 11.0 7.432831 101 0 100 12.0 7.819757 66 0 64 13.0 8.174548 64 0 66 14.0 8.499873 53 0 48 15.0 8.798179 42 0 43 16.0 9.07171 37 0 38 17.0 9.322523 36 0 34 18.0 9.552506 22 0 23 19.0 9.763388 24 0 23 20.0 9.956757 21 0 22 21.0 10.134065 17 0 16 22.0 10.296648 10 0 10 23.0 10.445727 16 0 16 24.0 10.582426 8 0 7 25.0 10.707771 9 0 9 26.0 10.822706 4 0 5 27.0 10.928096 7 0 6 28.0 11.024732 6 0 7 29.0 11.113343 8 0 9 30.0 11.194595 6 0 4 31.0 11.269098 7 0 8 32.0 11.337414 5 0 5 33.0 11.400056 8 0 8 34.0 11.457495 7 0 8 35.0 11.510164 5 0 3 36.0 11.558458 2 0 2 37.0 11.602742 3 0 4 38.0 11.643348 3 0 3 39.0 11.680581 2 0 4 40.0 11.714722 5 0 2 41.0 11.746028 0 0 0 42.0 11.774734 1 0 2 43.0 11.801055 3 0 2 44.0 11.825191 4 0 4 45.0 11.847322 0 0 0 46.0 11.867615 1 0 1 47.0 11.886222 1 0 1 48.0 11.903284 1 0 1 49.0 11.91893 1 0 2 50.0 11.933275 1 0 0 51.0 11.94643 0 0 1 52.0 11.958491 1 0 0 53.0 11.969551 1 0 3 54.0 11.979693 3 0 1 55.0 11.988992 1 0 1 56.0 11.997519 0 0 0 57.0 12.005338 1 0 1 58.0 12.012507 0 0 0 59.0 12.019081 0 0 0 60.0 12.025109 0 0 0 61.0 12.030636 1 0 1 62.0 12.035705 0 0 0 63.0 12.040352 0 0 0 64.0 12.044613 1 0 1 65.0 12.048521 0 0 0 66.0 12.052104 0 0 0 67.0 12.055389 0 0 0 68.0 12.058402 0 0 0 69.0 12.061164 0 0 0 70.0 12.063697 1 0 1 71.0 12.066019 1 0 1 72.0 12.068149 0 0 0 73.0 12.070102 0 0 0 74.0 12.071892 0 0 0 75.0 12.073534 1 0 1 76.0 12.07504 0 0 0 77.0 12.07642 0 0 0 78.0 12.077686 2 0 2 79.0 12.078847 0 0 0 80.0 12.079911 0 0 0 81.0 12.080887 0 0 0 82.0 12.081782 0 0 0 83.0 12.082602 1 0 1 84.0 12.083355 0 0 1 85.0 12.084045 1 0 0 86.0 12.084677 0 0 0 87.0 12.085257 0 0 0 88.0 12.085789 0 0 0 89.0 12.086277 1 0 1 90.0 12.086724 0 0 0 91.0 12.087134 1 0 1 92.0 12.08751 0 0 0 93.0 12.087855 0 0 0 94.0 12.088171 0 0 0 95.0 12.088461 0 0 0 96.0 12.088727 0 0 0 97.0 12.088971 0 0 0 98.0 12.089194 0 0 0 99.0 12.089399 0 0 0 100.0 12.089587 0 0 0