FastQCFastQC Report
Mon 17 Oct 2022
01B_S43_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename01B_S43_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521786
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCTGACTAATTCATGTCAATAAATCACATCAAATGCAAAAAAAAAA674311.4912470426508464No Hit
CTGTATTGAGCTAAAATAAACCAAATGGGATGAACAGTGAAAAAAAAAAA109550.24227152722397743No Hit
GAAAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108050.23895425391648345No Hit
AGGCATCCTGGCTTTGAAATAAAAACATGTTATTTACAAAAAAAAAAAAA94680.20938629116902038No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77440.17125976328822284No Hit
TCCTGGGGTCCACAAAAACAGAAACCATGACAAGTAACAAACACTGATAC70800.15657530011371615No Hit
CTTGCCTGGGAACCCAAAGCTAACAAGTGTATAAATCCTCTGTATTCACT69910.15460705128460303No Hit
TCTGCCTGACTAATTCATGTCAATAAATCACATCAAATGAAAAAAAAAAA68920.152417650901657No Hit
CACAAAATAAACACAATACAAAGAGGAAGAATAGGGCAAATAAATGACAA67260.14874653510803032No Hit
ACTGCCAGGGCAACAGTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAG65360.1445446555852046No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACAC57160.12641022817090417No Hit
CATGCATGGGCCTTCAAATAAAACAATTCAATTGAAAAAAAAAAAAAAAA55570.12289391846496053No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53150.11754205086220357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)