FastQCFastQC Report
Mon 17 Oct 2022
30B_S72_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30B_S72_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6937482
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT239230.34483693074807253No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA179440.25865292335172907No Hit
TGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAGGTA157590.2271573461379792No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG144540.2083464865206137No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA122370.17638964684881345No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG118040.17014818921331976No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT112960.16282564769177058No Hit
GAATGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAG104330.15038597577622545No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT97800.14097333874163567No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC95100.1370814367518359No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG92790.133751698382785No Hit
CATGTCAATAAATCACATCAAATGC87520.12615528227676842No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG82910.11951021999048068No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT82580.11903454308061628No Hit
AGGAGTTTAAAAGAAGATTTAAAAAAACGTATGTGTTGTCGCTGTGTGAT78520.11318227564410258No Hit
ATTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTC72760.10487955139919641No Hit
ACTGAATGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAAT72730.10483630804375421No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC72270.10417324326030684No Hit
ACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG72270.10417324326030684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)