FastQCFastQC Report
Mon 17 Oct 2022
29B_S71_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename29B_S71_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388499
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA139980.4131032648969352No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG81080.23927998798287972No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG72860.215021459354127No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT58650.17308548711391092No Hit
ACAAAATAAATTCCTACCTG48430.1429246400840018No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC39600.11686590434289636No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC38790.11447546539042804No Hit
GCTACATTAAAATCCATTGCAT36570.10792389196514446No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA36490.1076877992290982No Hit
CATGTCAATAAATCACATCAAATGC35620.10512029072459518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)