FastQCFastQC Report
Mon 17 Oct 2022
28B_S70_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename28B_S70_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115296
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA261770.6360903322628554No Hit
CAAGCTACTGATGCGACAATAAAAATATTTTCACTTC203940.4955658110619503No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG188070.4570023638639845No Hit
AATAAAAATATTTTCACTTC186390.4529200329696819No Hit
AACCAAGCTACTGATGCGACAATAAAAATATTTTCACTTC164820.40050582023747505No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG152450.3704472290693063No Hit
ACAAAATAAATTCCTACCTG122390.29740266556767725No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT104600.25417369734765133No Hit
GACAATAAAAATATTTTCACTTC95200.2313320840104819No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA93380.22690955887498734No Hit
AATACCGCAGCTAGGAATAATGGAATAGGACTCCGGTTCTATTTTGTGGG91230.22168514731382627No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA87300.21213540897179695No Hit
ATTACAAAATAAATTCCTACCTG83230.2022454763885757No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG82730.2010304969557475No Hit
CCAAACCAAGCTACTGATGCGACAATAAAAATATTTTCACTTC79300.19269573804654636No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC78010.18956109110984967No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA70690.1717737922132454No Hit
TCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTG70100.17034011648250819No Hit
TGCGACAATAAAAATATTTTCACTTC63620.15459398303305522No Hit
TGCTGTTCTATTACAAAATAAATTCCTACCTG62800.15260141676321703No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG61860.15031725542950009No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC58790.14285728171193518No Hit
CGGGGGCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA57750.1403301244916526No Hit
GACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCAACTAGCGA57250.13911514505882444No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCAACTAG55830.13566460346959247No Hit
AAATAAAGTCCTACGCAATTGT51920.12616346430487624No Hit
TCTATTACAAAATAAATTCCTACCTG49730.12084185438908891No Hit
CATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG49140.11940817865835167No Hit
CGCCTGAATACCGCAGCTAGGAATAATGGAATAGGACTCCGGTTCTATTT49050.11918948236044259No Hit
GCTACATTAAAATCCATTGCAT47190.11466975887032185No Hit
ATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA47110.11447536216106934No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC45950.111656609876908No Hit
GCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGA45130.10966404360706983No Hit
AGGAGTTTAAAAGAAGATTTAAAAAAACGTATGTGTTGTCGCTGTGTGAT42130.10237416701010084No Hit
CTGAATACCGCAGCTAGGAATAATGGAATAGGACTCCGGTTCTATTTTGT41810.10159658017309083No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT41230.10018720403101017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)