FastQCFastQC Report
Mon 17 Oct 2022
27B_S69_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename27B_S69_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905016
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA224780.45826557956181996No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG128920.26283298566202434No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT105850.2157994999404691No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG100170.20421951732675284No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT90060.1836079637660713No Hit
CATGTCAATAAATCACATCAAATGC67430.1374715189512124No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA62910.12825646236424101No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC58990.12026464337731009No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA50120.10218111419004546No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA49180.10026470861664875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)