FastQCFastQC Report
Mon 17 Oct 2022
25B_S67_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename25B_S67_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118806
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA230290.5591183464334081No Hit
GCTACATTAAAATCCATTGCAT150660.36578561845350327No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG142710.34648390820058045No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG125580.3048941853537166No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT115160.279595591537936No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC96220.23361139126241928No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT95220.23118350318029063No Hit
TAGGCTACATTAAAATCCATTGCAT93800.2277359021036679No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA70310.1707048110544658No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA68240.16567908272445947No Hit
GAAGATTGACATCTCGAAAGAGCTTTAACATTCATTCAACATTGTGCATG68000.1650963895847486No Hit
ACAAAATAAATTCCTACCTG65600.15926945818763982No Hit
CATGTCAATAAATCACATCAAATGC54600.1325626892842246No Hit
TTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACT52550.12758551871586085No Hit
TGCTAGGCTACATTAAAATCCATTGCAT52390.12719705662272027No Hit
ATTACAAAATAAATTCCTACCTG52300.12697854669532868No Hit
TCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTG51420.12484200518305547No Hit
AATAAAAATATTTTCACTTC49040.11906363154758927No Hit
CAAGCTACTGATGCGACAATAAAAATATTTTCACTTC47560.11547035718603886No Hit
AAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAAC47320.114887664046328No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA45090.10947347362318109No Hit
TGCTGTTCTATTACAAAATAAATTCCTACCTG42580.10337947453703815No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC41800.10148572183297781No Hit
AACCAAGCTACTGATGCGACAATAAAAATATTTTCACTTC41430.10058740324259022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)