FastQCFastQC Report
Mon 17 Oct 2022
24B_S66_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24B_S66_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873697
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA139850.2380953597027562No Hit
GTAAGGATTGTACACTTAGG130100.22149593348107674No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT112110.19086786397051125No Hit
CATGTCAATAAATCACATCAAATGC100190.17057400134872466No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA93460.15911614099263208No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC93210.15869051467925566No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG84100.1431806918198198No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGT77040.13116100473007034No Hit
AAAGTAAGGATTGTACACTTAGG76620.130445952523598No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG70420.11989041995186336No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG68690.1169450858632987No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC65140.11090119221335389No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA63860.10872198548886672No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT62850.10700245518282607No Hit
CACAAAGTAAGGATTGTACACTTAGG58990.10043078490429452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)