FastQCFastQC Report
Mon 17 Oct 2022
23B_S65_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename23B_S65_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6146725
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA348060.5662527606164258No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT331380.5391163587113463No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG220580.35885776572077No Hit
CATGTCAATAAATCACATCAAATGC185460.3017216485201469No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT157340.2559737095770512No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG141280.22984597488906697No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA134660.21907601202266247No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC127450.2073461884174093No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC124950.20327898189686378No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA118390.1926066319869524No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT110690.1800796359036723No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT109240.1777206561217559No Hit
GCTACATTAAAATCCATTGCAT94190.153236072868072No Hit
GAAGATTGACATCTCGAAAGAGCTTTAACATTCATTCAACATTGTGCATG93610.15229248095530548No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG88120.14336089543618757No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC79590.12948358678808633No Hit
AAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAAC77030.12531876731104774No Hit
AAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGT76860.12504219726765067No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG75780.123285164050775No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG75690.12313874461603537No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA73830.12011274296474952No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA73270.11920168870414732No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA73080.11889258100858588No Hit
CAGGGTATGTGTGTTTTGCCCACCAAATCCAGGGTCATGTTCAGTATGGA71440.11622449353110803No Hit
TTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACT70000.11388178257527382No Hit
AGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAA68010.11064428618491962No Hit
AAGGTCAAAGCATGCTAGATAGGAAGATTGACCTCTCGCAAGAGCTTTAA67440.10971696309823524No Hit
TTGAGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATG67190.10931024244618069No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC66880.10880590883763305No Hit
TTAAAATGTGTTTTTTTTTGTCTGACCCTGTGAATGATGTCAATGTACTG65980.10734171449023668No Hit
AAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCT65840.10711395092508612No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA65630.1067723055773603No Hit
AAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTT64910.1056009500994432No Hit
GAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAA62120.10106194762251443No Hit
ACTTTCTGTGACGTATATAAGGGCTGTGCTGACTGTTTACGCGCTCTCTG62120.10106194762251443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)