FastQCFastQC Report
Mon 17 Oct 2022
22B_S64_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename22B_S64_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4263841
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT111800.26220489929150737No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA108850.25528625481109635No Hit
CATGTCAATAAATCACATCAAATGC68600.16088780045972634No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG67500.15830796692465784No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC58230.1365670061336715No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA57160.13405753169501397No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC53610.1257317052863838No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG49920.11707753642783583No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT48530.11381756496079475No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA47340.11102665413649337No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC43750.10260701559931526No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG43420.1018330655387947No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA42820.10042588361057554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)