FastQCFastQC Report
Mon 17 Oct 2022
21B_S63_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename21B_S63_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4629100
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA293480.6339893283791666No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG175760.37968503596811476No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG131280.2835972435246592No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT117440.2536994232140157No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT101750.2198051457086691No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC79420.17156682724503683No Hit
CATGTCAATAAATCACATCAAATGC67460.14573027154306453No Hit
ACAAAATAAATTCCTACCTG66800.14430450843576503No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA65590.141690609405716No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA65100.14063208831090276No Hit
GCTACATTAAAATCCATTGCAT64130.13853664859259898No Hit
AGGAGTTTAAAAGAAGATTTAAAAAAACGTATGTGTTGTCGCTGTGTGAT60780.131299820699488No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC50760.10965414443412326No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGT46930.10138039791752176No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC46930.10138039791752176No Hit
ATTACAAAATAAATTCCTACCTG46490.10042988917932211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)