FastQCFastQC Report
Mon 17 Oct 2022
20B_S62_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename20B_S62_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292186
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA387520.5314181508809567No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG243790.3343167604337026No Hit
CATGTCAATAAATCACATCAAATGC220310.30211791087062234No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT183980.25229745922553265No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT177420.24330152851284922No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG151360.20756464522435386No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA113260.15531693788392123No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC109360.14996874736875881No Hit
GTAAGGATTGTACACTTAGG103910.14249499395654472No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC97960.13433557509366875No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC79010.10834885451358482No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA78120.10712837001140674No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG75720.10383717584822987No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGT73820.10123164713571485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)