FastQCFastQC Report
Mon 17 Oct 2022
19B_S61_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename19B_S61_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220025
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA167480.3208413752807697No Hit
CATGTCAATAAATCACATCAAATGC140310.26879181613114883No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG103590.19844732544384366No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG103290.19787261555260752No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC85980.16471185482828146No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT73120.14007595749062504No Hit
GCTACATTAAAATCCATTGCAT71810.1375663909655605No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT69360.1328729268537986No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT69330.13281545586467497No Hit
AATAAAAATATTTTCACTTC67350.12902237058251637No Hit
ACAAAATAAATTCCTACCTG65660.12578483819521935No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA59420.11383087245750739No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG56560.10835197149438941No Hit
TGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCA56460.108160401530644No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA52580.1007274869373231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)