FastQCFastQC Report
Mon 17 Oct 2022
18B_S60_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename18B_S60_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928603
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA316700.45709070067948765No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT213850.30864807811906675No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG196300.2833182966320916No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT144100.2079784337477555No Hit
CATGTCAATAAATCACATCAAATGC96840.13976843528197533No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA95670.13807978318284364No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG90900.13119527847099913No Hit
GCTACATTAAAATCCATTGCAT84950.12260768873609874No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC70870.10228613185082189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)