FastQCFastQC Report
Mon 17 Oct 2022
17B_S59_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename17B_S59_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852916
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA229180.47225214695659273No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG131050.2700438251970568No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT93230.19211129968043955No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG79790.1644166105492038No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG76980.15862627747935468No Hit
CATGTCAATAAATCACATCAAATGC65070.134084331976898No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC61690.12711944735907235No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC58060.1196394085535377No Hit
TGATTGTTAAAATAACATTGGGAATCTCTCTG54020.1113145168801603No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG52580.10834722875895646No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT51820.10678116002832112No Hit
ACAAAATAAATTCCTACCTG50830.10474114944499348No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA49020.10101143312598033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)