FastQCFastQC Report
Mon 17 Oct 2022
16B_S58_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename16B_S58_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779972
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA132940.2781187839594039No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG75100.15711389104371323No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT58170.12169527352879893No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA57410.12010530605618609No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG55700.11652787924280729No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT55670.11646511736888834No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT51370.10746924877384219No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC49990.10458220257357156No Hit
CATGTCAATAAATCACATCAAATGC48110.10064912514131882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)