FastQCFastQC Report
Mon 17 Oct 2022
15B_S57_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15B_S57_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4846378
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA235030.48496010835308345No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT205940.42493590058389996No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG146350.3019780958068066No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT102870.21226161062962898No Hit
CATGTCAATAAATCACATCAAATGC83210.17169523301731726No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA78040.16102747247532073No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC61370.1266306507664074No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA58000.11967700414618916No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT57380.11839769823979887No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG54720.11290906322205985No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC54720.11290906322205985No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA54090.11160912334943746No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT53760.1109282024637781No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG51850.1069871149134467No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC48470.10001283432699637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)