FastQCFastQC Report
Mon 17 Oct 2022
14B_S56_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename14B_S56_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825034
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA344090.43972971874627No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT335850.4291994130632531No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG216630.27684224758640025No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT147240.18816531659798538No Hit
CATGTCAATAAATCACATCAAATGC139640.1784528987350087No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG138060.1764337381792846No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA134160.17144973427591498No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA125750.16070217714069995No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC113430.1449578366049272No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG108520.13868310348555674No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC101840.13014639936388775No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG90580.11575668553005648No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG90150.11520716715096702No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA88400.11297075514304475No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA85540.10931581894724036No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC82670.10564810325424784No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC82540.10548196979080218No Hit
GAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAA79710.10186537208656217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)