FastQCFastQC Report
Mon 17 Oct 2022
13B_S55_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename13B_S55_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7900878
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT327110.4140172775734545No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA320070.4051068754637143No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG206470.26132538687472456No Hit
CATGTCAATAAATCACATCAAATGC177010.22403839168254466No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA144750.18320748656035443No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG144320.18266324324967428No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT143040.1810431701388124No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG122530.1550840299014869No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC114200.14454089785970622No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA109110.13809857587979463No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC108970.13792138038329413No Hit
ACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCCAATG98860.12512533417172117No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA97000.12277116543250002No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG93760.11867035537063096No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT87720.11102563537875158No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT86500.10948150319496136No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA85750.10853224160656576No Hit
AGGAGTTTAAAAGAAGATTTAAAAAAACGTATGTGTTGTCGCTGTGTGAT81660.10335560174451498No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA81070.1026088492949771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)