FastQCFastQC Report
Mon 17 Oct 2022
12B_S54_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename12B_S54_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5831130
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT164390.28191791299456537No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA113020.19382178068401834No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA83890.1438657687275022No Hit
CATGTCAATAAATCACATCAAATGC73390.12585896730136353No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG68900.11815891602485282No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA68760.11791882533917096No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC64940.1113677794869948No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC63470.10884682728733538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)