FastQCFastQC Report
Mon 17 Oct 2022
11B_S53_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename11B_S53_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4087601
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAATAAATCACATCAAATGC99770.24407959583139352No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT63040.1542224889366648No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG59090.14455911915081732No Hit
TGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAGGTA55320.1353361054564768No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA54850.13418628677309746No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT52180.1276543380824107No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC47390.11593597320286397No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT46780.11444365533720147No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC45370.11099419928706349No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG43710.10693313755427694No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC41950.10262743354843099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)