FastQCFastQC Report
Mon 17 Oct 2022
10B_S52_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename10B_S52_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097455
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA155310.3046814537842904No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG93070.1825813077310148No Hit
CATGTCAATAAATCACATCAAATGC82060.16098229410558798No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC80800.1585104723827871No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT70470.13824545778236394No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT65650.1287897588110145No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC60530.11874553085804583No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT58290.11435118112862203No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA54540.1069945688583813No Hit
GTAAGGATTGTACACTTAGG53780.10550362877161251No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG52700.10338492443778316No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC52150.10230595463814787No Hit
GAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAA51030.10010877977343596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)