FastQCFastQC Report
Mon 17 Oct 2022
09B_S51_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename09B_S51_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7449861
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA304980.40937676555307545No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT234530.31481124278694594No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG190680.2559510841880137No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT141880.1904465063173662No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA116790.15676802560477301No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT110420.1482175305015758No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT97310.13061988673345717No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG86860.11659277938205825No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA81470.1093577450639683No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC79440.10663286200910327No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG77520.10405563271583189No Hit
CATGTCAATAAATCACATCAAATGC76940.10327709470015616No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC76430.10259251816913094No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC75970.10197505698428468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)