FastQCFastQC Report
Mon 17 Oct 2022
08B_S50_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename08B_S50_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261529
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT155280.36437626025776193No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA95250.22351132656846873No Hit
TGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAGGTA85430.20046795410755153No Hit
CATGTCAATAAATCACATCAAATGC76990.18066285598431925No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA61430.14415013953911845No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG58530.13734507027876613No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG56380.13229993272367735No Hit
ACTTTCTGTGACGTATATAAGGGCTGTGCTGACTGTTTACGCGCTCTCTG49170.11538112259707725No Hit
GAATGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAG49050.11509953352423508No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC48580.1139966429889366No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA47150.1106410398709008No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT44680.10484499812156622No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG43890.10299120339202197No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG43270.10153632651567078No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA42740.10029264144395121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)