FastQCFastQC Report
Mon 17 Oct 2022
06B_S48_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename06B_S48_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5705636
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT168700.295672559553396No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG130210.22821294593626373No Hit
CATGTCAATAAATCACATCAAATGC104720.18353782119995035No Hit
GTAAGGATTGTACACTTAGG94570.16574839334300331No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA92600.16229566695106382No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA75950.13311399465370732No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC72200.12654154593808647No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA68640.12030210129072376No Hit
CTTGTGATACCAGCTGTTGTCAGTGAGGCCTGTGTATGACATCACAATGC66730.11695453407823421No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA61670.10808610994462318No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT60320.10572002840699968No Hit
AAAGTAAGGATTGTACACTTAGG58020.10168892652808556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)