FastQCFastQC Report
Mon 17 Oct 2022
05B_S47_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename05B_S47_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6469155
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA215680.3333974839063216No Hit
TGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAGGTA200260.30956129509959185No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT129020.19943872113127606No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG126870.19611525771140126No Hit
GTAAGGATTGTACACTTAGG125220.1935646927612648No Hit
GAATGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAATCAG120730.1866240645030147No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA109210.1688164837602438No Hit
GCTGGCGGTCCGCCGCGAGGCGAGCTACCGCCTGTCCCAGCCCCTGCCTC107700.16648233038163407No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT89030.1376223015216052No Hit
ACTGAATGTTCATGGACAAAAACAAACGACCGGCTAACTTCTGAACCAAT83990.12983148494664296No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG80480.12440573768907993No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC76180.1177588108493304No Hit
AAAGTAAGGATTGTACACTTAGG75330.11644488345077526No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA74310.11486817057250909No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT65470.10120332562753559No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG65390.10107966187237746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)