FastQCFastQC Report
Mon 17 Oct 2022
04B_S46_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename04B_S46_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739113
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAATAAATCACATCAAATGC488291.0303404877663815No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT234160.49410090031615617No Hit
GTAAGGATTGTACACTTAGG206660.4360731639021901No Hit
AAAGTAAGGATTGTACACTTAGG109720.23152011779419482No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG93760.19784292967903486No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC93050.19634475902980159No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA86840.18324104109777506No Hit
CACAAAGTAAGGATTGTACACTTAGG86220.18193277940407837No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT81100.17112906993355087No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC79640.16804832465484573No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC76700.16184463210731628No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA74620.15745562513491448No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG73090.15422717289079202No Hit
CATGTCAATAAATCACATCAAATG66490.14030051615144015No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGT64950.13705096291225805No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA64540.13618582211481345No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA61610.13000323056234364No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT60470.1275977171255465No Hit
AAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGT58880.12424265891106626No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA56990.12025457084479732No Hit
AAACAGAAACCATGACAAGTAACAAACACTGATACACTGATAGATCAGAA55460.11702611860067486No Hit
TTGAGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATG54620.11525363501566643No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC53720.11335454546030027No Hit
AAGGTCAAAGCATGCTAGATAGGAAGATTGACCTCTCGCAAGAGCTTTAA52070.10987288127546231No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG52000.10972517431004494No Hit
ATAAACCAAATGGGATGAACAGTG51760.10921875042861397No Hit
AGTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTG50770.10712975191771118No Hit
TGTGTTTAAAAATAATAATTTGCTTATTATTATAAACATTTTTATTACAG49380.10419671360442345No Hit
GTGAAACTATTACAAATCCTGTTATGTCATTTATGCTGTTCTATTACAAAATAAATTCCTACCTG48480.10229762404905729No Hit
GTAAGGATTGTACACTTAGGAAG47540.10031413051345263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)