FastQCFastQC Report
Mon 17 Oct 2022
03B_S45_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename03B_S45_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6359398
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT459510.7225683940523929No Hit
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA239230.37618340603937667No Hit
CATGTCAATAAATCACATCAAATGC226080.3555053481477335No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC221390.34813043624569495No Hit
GAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTA184250.28972868186579925No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATG147570.23205026639313972No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG146630.2305721390609614No Hit
AAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGT138400.21763066252497484No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT117260.18438852230981612No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT115710.18195118468760724No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA104580.16444952808426205No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT104540.16438662904885024No Hit
AAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTT104080.16366329014161404No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC104010.1635532168296433No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA101660.15985789849919757No Hit
AAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCT96820.15224711521436463No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA96570.1518539962430406No Hit
AGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAA93820.14752968755847645No Hit
AACAAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGT89400.14057934414546785No Hit
TTGAGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATG87840.138126281764406No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC86770.1364437325671392No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG85060.13375479880328295No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA83960.1320250753294573No Hit
GAGAGAAGGATGGGGAGTGAGTGACAGAGAGATAGAGAGAGACATTGCTG83860.13186782774092767No Hit
AAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAATTATTATTA80840.12711895056733358No Hit
GGGGAAACAAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACC78700.12375385217280001No Hit
AAGGTCAAAGCATGCTAGATAGGAAGATTGACCTCTCGCAAGAGCTTTAA78650.1236752283785352No Hit
GAAGATTGACATCTCGAAAGAGCTTTAACATTCATTCAACATTGTGCATG74910.11779416856752795No Hit
GAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAA74420.11702365538373287No Hit
AGTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTG74400.11699220586602695No Hit
ATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTACTGCC72500.11400450168396442No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC71860.11299811711737494No Hit
TTAAAATGTGTTTTTTTTTGTCTGACCCTGTGAATGATGTCAATGTACTG71590.112573548628345No Hit
AGGAGTTTAAAAGAAGATTTAAAAAAACGTATGTGTTGTCGCTGTGTGAT71310.11213325538046212No Hit
CAGGGTATGTGTGTTTTGCCCACCAAATCCAGGGTCATGTTCAGTATGGA71220.11199173255078546No Hit
AGGAGTTTAAAAGAAGATTTTAAAAAACGTATGTGTTGTCGCTGTGTGAT67320.10585907659813082No Hit
AAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAACACTTT66600.10472689396071766No Hit
GGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGT65150.10244680392703838No Hit
AAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTG65070.1023210058562147No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG63690.10015098913450614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)