FastQCFastQC Report
Mon 17 Oct 2022
02B_S44_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename02B_S44_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134180
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAGGTA104350.25240797449554686No Hit
CATGTCAATAAATCACATCAAATGC90160.2180843601391328No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT71770.1736015364594672No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTAAACCTGATGTCTTAAT69980.16927177820027187No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG67410.163055309638187No Hit
GAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAATCAG64740.15659695513983427No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA63490.1535733809364856No Hit
AGTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTG57350.13872158444963695No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC55900.1352142383737525No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG51390.12430518264807049No Hit
AAACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAA51020.12341020468387928No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT49910.12072527079130566No Hit
TAAATTCATGCACTCAATTTAGTAAATCAGTTACTGCAATCAAGGAGGTA49480.11968516126535371No Hit
AACTGACGCTATTAGGTAGGAATGTTAAGTAGGAATTTACCGTATTAAAC46820.11325099536062773No Hit
GTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGA43370.10490593055938542No Hit
GAGAGAAGGATGGGGAGTGAGTGACAGAGAGATAGAGAGAGACATTGCTG42920.1038174438461799No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG42910.1037932552525531No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA42670.10321272900551015No Hit
ACTGAATGTTCATGGACAAAAACAAACCACCGGCTAACTTCTGAACCAAT42180.10202748791779748No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC41740.10096318979821875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)