FastQCFastQC Report
Mon 17 Oct 2022
01B_S43_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename01B_S43_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487092
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAATAAATCACATCAAATGC716192.053831674071117No Hit
ACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCT173260.49686099477731016No Hit
ATAAACCAAATGGGATGAACAGTG117100.33580989546590684No Hit
GAAATAAAAACATGTTATTTAC115130.33016048902638645No Hit
GTAAGGATTGTACACTTAGG108120.3100577788025094No Hit
ATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCC104930.3009097551770931No Hit
GAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTGATGTCTT99480.285280686600755No Hit
GTAACAAGTTCTACTCCAAGGGGGAGTGTGACGAGTACTGTGGGGTGATG96410.2764767892559187No Hit
ACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCTT83890.24057294731541354No Hit
AGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCA83260.23876628434236893No Hit
GAGAGAGAAAAGCGATGTTGTTGTTTCAAACCTGAACCTGATGTCTTAAT82600.23687358979917936No Hit
AAACAGAAACCATGACAAGTAACAAACACTGATACACTGATAGATCAGAA81270.23305952352275194No Hit
TGTGTTTAAAAATAATAATTTGCTTATTATTATAAACATTTTTATTACAG78250.2243990121281572No Hit
AACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATATTTGAGC76970.22072833180197138No Hit
CATGTCAATAAATCACATCAAATG73950.2120678204073767No Hit
GAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATC73240.2100317399139455No Hit
TTGAGAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATG70780.2029771511620571No Hit
GAGACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTC70110.20105577942881922No Hit
AAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGT68740.19712700439219843No Hit
ACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATATTTGAGCT66580.19093273134175984No Hit
AAGGTCAAAGCATGCTAGATAGGAAGATTGACCTCTCGCAAGAGCTTTAA65360.18743411415586397No Hit
GACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCATCT62860.18026481664378227No Hit
AGTGAAACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATATT61390.17604926970667822No Hit
AAAGTAAGGATTGTACACTTAGG61390.17604926970667822No Hit
AAAGTCAAATCATGGTAGATAGGAAGATTGACATCTCGAAAGAGCTTTAA60080.1722925578103474No Hit
GTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTAG59980.17200578590986415No Hit
TACTGTGTTTAAAAATAATAATTTGCTTATTATTATAAACATTTTTATTA59900.17177636838947755No Hit
GAGAGAAGGATGGGGAGTGAGTGACAGAGAGATAGAGAGAGACATTGCTG59250.16991235103633628No Hit
GAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCTGATTTCG57560.16506590591816905No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTGT56370.16165332030241816No Hit
ATACAAAGAGGAAGAATAGGGCAAATAAATGACAAATAAACACGACTTTTTAAAAATG55990.16056358708058177No Hit
GTCGAGACTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACG52770.15132953188502055No Hit
AGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGAGTA52280.14992434957265252No Hit
AAAGAAGATTTTAAAAAACGTATGTGTTGTCGCTGTGTGATGTGATGATG52060.1492934513915893No Hit
CTCAATCAAAAAAGCTAATATGCATCGAAACCCATTAAATGCACCATCAT51870.1487485847806711No Hit
TTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTCG51210.14685589023748155No Hit
TACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCCCCT51070.14645440957680497No Hit
TGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTTC51060.14642573238675666No Hit
AGGAGTTTAAAAGAAGATTTTAAAAAACGTATGTGTTGTCGCTGTGTGAT49230.14117780660791282No Hit
GTGGGAGTGAAACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACT49060.14069029437709127No Hit
AGTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTG47370.13584384925892404No Hit
AAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAACACTTT47240.1354710457882958No Hit
GAAATAAAAACAATGTTATTTACAAC46970.134696761656991No Hit
GAAACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATATTTGA46320.13283274430384975No Hit
GAGTGAAACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATAT46090.13217316893273823No Hit
GGCTGTGATATCAGCCGACAGCGACTTGCTGTTTAGCACTTGGTGATTGA45640.13088269538056352No Hit
AGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAACA42850.12288175935708034No Hit
AAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACACATCC42350.121447899854664No Hit
ATTTGGCATCAGCTCTCAGACTGTAGTGGTGGCTGCACGCCACATGGGTC42200.12101774200393911No Hit
GAAGAGAAATGCACAATGCAAAACACATCCCCTCCCGGTTTCTATGTCAA41940.12027213506268261No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGTACTACTATTAAATACATATCACC41450.11886695275031459No Hit
AACAAATGGTGCATGATGCAAGAGGAAAAGAGAGAGAAAAGCGATGTTGT41030.11766251076828486No Hit
GGATTGGGACCATGCTGACCATGCAAAGTGTCTACCCAGGGATCACTTCT40590.11640071440615848No Hit
GTGACGAGTACTGTGGGGTGATGAAGGATGGACAAACTGAGTTCCTGAAA40380.11579849341514363No Hit
ACAAAGGTCAAAGCATGCTAGATAGGAAGATTGACCTCTCGCAAGAGCTT39770.11404918482219568No Hit
GGGATAGCACTACCCAAGAAGTAGTTCCTGGATATTGGAGACTCCTAAAC39240.11252929374963437No Hit
CACAAAGTAAGGATTGTACACTTAGG39120.11218516746905444No Hit
TGAAACAATATTTGATTGTTTTGATGAGGCTGCAGCAGTTACTATATTTG38980.11178368680837787No Hit
AGGAAAAGAGAGAGAAAAGCGATGTTGTTGTTGTTTCAAACCTGAACCTG38940.11166897804818456No Hit
GGGGAAATACCTGAAGGTGATTGAGAAGAGAAATGCACAATGCAAAACAC37800.1083997783826753No Hit
AGAAGAAATGGAGAATTTGGAGGTGGAGAATGTGTAAAATACAAAGGCTG36910.10584750846837421No Hit
TAAGACGGTCTCTGATTTCGTCAAAGCTGGAGGCACCGACTACAGCCTCA36670.10515925590721437No Hit
CTGGAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCTG36390.10435629458586122No Hit
GATGGGGAGTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGA36040.1033525929341698No Hit
AAAGCTAACAAGTGTATAAATCCTCTGTATTCACTTTGCTGGATATTTGT35880.10289375789339655No Hit
AGGGCTGTGATATCAGCCGACAGCGACTTGCTGTTTAGCACTTGGTGATT35710.102406245662575No Hit
GTGAGTGACAGAGAGATAGAGAGAGACATTGCTGACTGTTGATTCTGTGA35480.1017466702914635No Hit
GTGAAACTATTACAAATCCTGTTATGTCATTTATGCTGTTCTATTACAAAATAAATTCCTACCTG35340.10134518963078691No Hit
GAAGAAAGTTGAAACCAAAAACTTTAAAGGGAGTAAGACGGTCTCTGATT35070.10057090549948208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)