FastQCFastQC Report
Sun 7 Aug 2022
05C_S89_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename05C_S89_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998004
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45600.1521011979970674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACACG43300.1444294270454609No Hit
AATGCCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCC36220.1208137147248636No Hit
ACCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG33570.1119745003675779No Hit
TGCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG32300.10773834858125607No Hit
AGCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG30210.10076704367305714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)