FastQCFastQC Report
Sun 7 Aug 2022
05C_S89_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename05C_S89_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997397
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47300.15780358757948978No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACACG45820.1528659700400047No Hit
AATGCCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCC34990.11673462007201582No Hit
ACCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG31090.10372333060985917No Hit
TGCTGGGGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG30670.10232211482162691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)